Hotspot

psbT-psbN

LSC · 71461-71512 · 52 bp

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Pi 0.0205
MarkerSeek score 47.80
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
52
variable_sites
2
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 CCCCCTAAGGTTCCGACTAA TATATACCGCGTTTGGGCAA 55.0 45.0 58.1 57.4 144 bp 1.0 75.1
psbT-psbN_p2 CCCCCTAAGGTTCCGACTAA TTATATACCGCGTTTGGGCA 55.0 45.0 58.1 57.4 145 bp 1.0 75.1
psbT-psbN_p3 TCTTCCGAGAACCCCCTAAG TATATACCGCGTTTGGGCAA 55.0 45.0 58.1 57.4 155 bp 1.0 75.0
psbT-psbN_p4 TCTTCCGAGAACCCCCTAAG TTATATACCGCGTTTGGGCA 55.0 45.0 58.1 57.4 156 bp 1.0 75.0
psbT-psbN_p5 TTCTTCCGAGAACCCCCTAA TATATACCGCGTTTGGGCAA 50.0 45.0 57.7 57.4 156 bp 1.0 73.4