Hotspot

ndhJ-ndhK

LSC · 49842-49943 · 102 bp

Back to Zoysia

Pi 0.00327
MarkerSeek score 45.80
Primer available yes
Label ndhJ-ndhK

Feature Metadata

label_name
ndhJ-ndhK
feature_type
igs
region
LSC
length_bp
102
variable_sites
1
pi
0.00327
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-ndhK_p1 GGTGATCGAAGCCCAAAGAT CCGGAACTTACGAGCAAGAA 50.0 50.0 58.0 57.9 285-288 bp 1.0 80.2
ndhJ-ndhK_p2 TGAACCACCTCATGCTTGAC CCGGAACTTACGAGCAAGAA 50.0 50.0 58.1 57.9 262-265 bp 1.0 79.8
ndhJ-ndhK_p3 GAATCCCAATCCCCTGCTTT CCGGAACTTACGAGCAAGAA 50.0 50.0 57.8 57.9 328-331 bp 1.0 79.1
ndhJ-ndhK_p4 GAACCACCTCATGCTTGACT CCGGAACTTACGAGCAAGAA 50.0 50.0 57.8 57.9 261-264 bp 1.0 79.1
ndhJ-ndhK_p5 GATAACCATCCCTGCTGCAT CCGGAACTTACGAGCAAGAA 50.0 50.0 57.7 57.9 232-235 bp 1.0 78.5