Hotspot

trnC-GCA-rpoB

LSC · 19110-20271 · 1162 bp

Back to Zoysia

Pi 0.00132
MarkerSeek score 47.10
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1162
variable_sites
4
pi
0.00132
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 TAGAGGATTTGCAGTCCCCT CCGGGTATTGTGGACATTCC 50.0 55.0 57.7 58.3 1244-1247 bp 1.0 75.7
trnC-GCA-rpoB_p2 TAGAGGATTTGCAGTCCCCT CGGGTATTGTGGACATTCCC 50.0 55.0 57.7 58.3 1243-1246 bp 1.0 75.7
trnC-GCA-rpoB_p3 TAGAGGATTTGCAGTCCCCT AAGTTCTTCTGCCAAGCCTT 50.0 45.0 57.7 57.6 1308-1311 bp 1.0 75.3
trnC-GCA-rpoB_p4 TAGAGGATTTGCAGTCCCCT AGTTCTTCTGCCAAGCCTTT 50.0 45.0 57.7 57.6 1307-1310 bp 1.0 75.3
trnC-GCA-rpoB_p5 TAGAGGATTTGCAGTCCCCT CAAGTTCTTCTGCCAAGCCT 50.0 50.0 57.7 58.4 1309-1312 bp 1.0 75.2