Hotspot

rps16-trnQ-UUG

LSC · 5565-6819 · 1255 bp

Back to Zoysia

Pi 0.00127
MarkerSeek score 34.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1255
variable_sites
3
pi
0.00127
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1328-1332 bp 1.0 78.6
rps16-trnQ-UUG_p2 TATCTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 1338-1342 bp 1.0 75.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1332-1336 bp 1.0 73.8
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1320-1324 bp 1.0 73.4
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1327-1331 bp 1.0 72.8