Hotspot

ycf2

IRa · 137821-143265 · 5445 bp

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Pi 0.0165

MarkerSeek score 44.50

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 5445
variable_sites: 223
pi: 0.0165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGAAACCAGAAGTGACGTGG	CCTTTTCTACGGATTCGCCA	50.0	50.0	58.1	58.0	874-883 bp	0.8	72.4
ycf2_p2	TCAGCCAATTGTCTCGATCG	CCTTTTCTACGGATTCGCCA	50.0	50.0	58.1	58.0	777-786 bp	0.8	72.4
ycf2_p3	TGAAACCAGAAGTGACGTGG	GCGGGATCTATTCACTCGAC	50.0	55.0	58.1	57.9	933-942 bp	0.8	72.1
ycf2_p4	GATCTCGGACCTACGAATGC	CCTTTTCTACGGATTCGCCA	55.0	50.0	57.9	58.0	494 bp	0.8	71.6
ycf2_p5	TTCCAAATGTTCCTGGCTCC	CCTTTTCTACGGATTCGCCA	50.0	50.0	58.1	58.0	326 bp	0.8	71.2