Hotspot

rpl16

LSC · 80764-82136 · 1373 bp

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Pi 0.0262

MarkerSeek score 57.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1373
variable_sites: 86
pi: 0.0262
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAGGGTCTGAGGTTGGATCA	TTAGAGAGGGCAGAGTTCCC	50.0	55.0	58.0	58.1	1770-1805 bp	1.0	86.4
rpl16_p2	AAGGGTCTGAGGTTGGATCA	ATTAATGGAGGGTCGAACGC	50.0	50.0	58.0	58.1	2057-2092 bp	1.0	86.2
rpl16_p3	CATATTGGGCACTGCTTCCT	ATTAATGGAGGGTCGAACGC	50.0	50.0	57.9	58.1	2186-2221 bp	1.0	85.8
rpl16_p4	AAGGGTCTGAGGTTGGATCA	CCCTACAAACTATTCGCGCT	50.0	50.0	58.0	58.1	1752-1787 bp	0.8	74.4
rpl16_p5	CATATTGGGCACTGCTTCCT	CCCTACAAACTATTCGCGCT	50.0	50.0	57.9	58.1	1881-1916 bp	0.8	74.0