Hotspot

accD

LSC · 57514-58935 · 1422 bp

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Pi 0.0345

MarkerSeek score 63.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 120
pi: 0.0345
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCTTGAAATACCGGGGTCCT	ATGTGACCCAAGAAAACCCC	50.0	50.0	58.0	58.0	1756 bp	0.6	63.8
accD_p2	TCTTGAAATACCGGGGTCCT	GTGACCCAAGAAAACCCCAT	50.0	50.0	58.0	58.0	1754 bp	0.6	63.8
accD_p3	GGTTCGAATCCGTATAGCCC	GTGACCCAAGAAAACCCCAT	55.0	50.0	57.9	58.0	2244-2247 bp	0.6	63.7
accD_p4	AGCTAGGACCCCTCCTTTTT	ATGTGACCCAAGAAAACCCC	50.0	50.0	57.9	58.0	1775 bp	0.6	63.6
accD_p5	AGCTAGGACCCCTCCTTTTT	GTGACCCAAGAAAACCCCAT	50.0	50.0	57.9	58.0	1773 bp	0.6	63.6