Hotspot

ndhG-ndhI

SSC · 113325-113767 · 443 bp

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Pi 0.0454

MarkerSeek score 70.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 443
variable_sites: 46
pi: 0.0454
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACAAACCAGCGAAGTGACC	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.1	57.9	915-921 bp	1.0	85.7
ndhG-ndhI_p2	TACAAACCAGCGAAGTGACC	GGTACATCGGTCAGGGTTTC	50.0	55.0	58.1	58.0	1268-1274 bp	0.8	72.4
ndhG-ndhI_p3	CCATCGAGTATTCGGAACCC	GGTACATCGGTCAGGGTTTC	55.0	55.0	57.8	58.0	1220-1226 bp	0.8	72.2
ndhG-ndhI_p4	TACAAACCAGCGAAGTGACC	ACATCGGTCAGGGTTTCATG	50.0	50.0	58.1	57.9	1265-1271 bp	0.8	71.7
ndhG-ndhI_p5	CTCGTACAAACCAGCGAAGT	GGTACATCGGTCAGGGTTTC	50.0	55.0	58.2	58.0	1272-1278 bp	0.8	71.6