Hotspot

ccsA-ndhD

SSC · 109664-109854 · 191 bp

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Pi 0.0524

MarkerSeek score 67.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 191
variable_sites: 23
pi: 0.0524
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCATTAGCCTGGGGTTTACC	ATTTTGGCCCACGAGAACTT	55.0	45.0	57.9	58.0	615-628 bp	1.0	84.8
ccsA-ndhD_p2	CCATTAGCCTGGGGTTTACC	TCCATCCCAATGCCAAAACA	55.0	45.0	57.9	58.0	880-893 bp	1.0	84.3
ccsA-ndhD_p3	CCATTAGCCTGGGGTTTACC	GCCTATGGGTTGGTTCGAAT	55.0	50.0	57.9	57.9	1210-1223 bp	1.0	83.5
ccsA-ndhD_p4	GATTCGCCACTTCAGGTCTT	ATTTTGGCCCACGAGAACTT	50.0	45.0	57.9	58.0	951-958 bp	0.8	72.1
ccsA-ndhD_p5	CCATTAGCCTGGGGTTTACC	TCCATCTGCATTCTTCTGCC	55.0	50.0	57.9	58.0	595-602 bp	0.8	71.9