Hotspot

ycf1-trnL-UAG

IRb · 107208-108536 · 1329 bp

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Pi 0.0408

MarkerSeek score 68.50

Primer available yes

Label ycf1-trnL-UAG

Feature Metadata

label_name: ycf1-trnL-UAG
feature_type: igs
region: IRb
length_bp: 1329
variable_sites: 109
pi: 0.0408
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnL-UAG_p1	CGGGAAGAACATAGCAGCAA	CAGCGTGTCTACCAATTCCA	50.0	50.0	58.3	57.9	1744-1907 bp	0.8	73.0
ycf1-trnL-UAG_p2	CGGGAAGAACATAGCAGCAA	AGCGTGTCTACCAATTCCAC	50.0	50.0	58.3	57.9	1743-1906 bp	0.8	73.0
ycf1-trnL-UAG_p3	ACATAGCAGCAACAACAGGT	CAGCGTGTCTACCAATTCCA	45.0	50.0	57.7	57.9	1736-1899 bp	0.8	72.9
ycf1-trnL-UAG_p4	ACATAGCAGCAACAACAGGT	AGCGTGTCTACCAATTCCAC	45.0	50.0	57.7	57.9	1735-1898 bp	0.8	72.9
ycf1-trnL-UAG_p5	CGGGAAGAACATAGCAGCAA	AAGACCTGAAGTGGCGAATC	50.0	50.0	58.3	57.9	2238-2403 bp	0.8	72.3