Hotspot

rpl22-trnH-GUG

IRb · 83251-83832 · 582 bp

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Pi 0.044

MarkerSeek score 73.90

Primer available yes

Label rpl22-trnH-GUG

Feature Metadata

label_name: rpl22-trnH-GUG
feature_type: igs
region: IRb
length_bp: 582
variable_sites: 59
pi: 0.044
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-trnH-GUG_p1	GCGTTCGACCCTCCATTAAT	GAGGGTAAATCGGCCACATT	50.0	50.0	58.1	57.9	1556-1577 bp	1.0	87.8
rpl22-trnH-GUG_p2	CCGTGTTCATTTCGGCTCTA	GATCCACTTGGCTACATCCG	50.0	55.0	58.0	58.1	718-726 bp	0.8	73.9
rpl22-trnH-GUG_p3	CCGTGTTCATTTCGGCTCTA	CCCCTTGGGGTTATCCTACA	50.0	55.0	58.0	58.1	880-888 bp	0.8	73.6
rpl22-trnH-GUG_p4	CCGTGTTCATTTCGGCTCTA	GAGGGTAAATCGGCCACATT	50.0	50.0	58.0	57.9	1121-1129 bp	0.8	73.5
rpl22-trnH-GUG_p5	CCGTGTTCATTTCGGCTCTA	AAAGAGGGTAAATCGGCCAC	50.0	50.0	58.0	57.9	1124-1132 bp	0.8	72.9