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atpE-trnM-CAU

LSC · 55647-55831 · 185 bp

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Pi 0.0422

MarkerSeek score 67.60

Primer available yes

Label atpE-trnM-CAU

Feature Metadata

label_name: atpE-trnM-CAU
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 18
pi: 0.0422
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-trnM-CAU_p1	ACGCGAGTCGAATCTATCAA	ACGGCAGAAGTCATTGGTTC	45.0	50.0	56.9	58.5	250-255 bp	1.0	74.4
atpE-trnM-CAU_p2	ACGCGAGTCGAATCTATCAA	CGGCAGAAGTCATTGGTTCA	45.0	50.0	56.9	58.5	249-254 bp	1.0	74.4
atpE-trnM-CAU_p3	ACGCGAGTCGAATCTATCAA	TGCTTTCATACGGCAGAAGT	45.0	45.0	56.9	57.5	259-264 bp	1.0	74.2
atpE-trnM-CAU_p4	ACGCGAGTCGAATCTATCAA	TACGGCAGAAGTCATTGGTT	45.0	45.0	56.9	57.1	251-256 bp	1.0	71.4
atpE-trnM-CAU_p5	ACGCGAGTCGAATCTATCAAT	TGCTTTCATACGGCAGAAGT	42.9	45.0	57.4	57.5	259-264 bp	1.0	70.2