Hotspot

trnS-GCU-trnG-UCC

LSC · 7737-8481 · 745 bp

Back to Zostera

Pi 0.0298
MarkerSeek score 65.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
745
variable_sites
50
pi
0.0298
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 811-823 bp 1.0 76.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 810-822 bp 1.0 76.7
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 811-823 bp 1.0 76.7
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 810-822 bp 1.0 76.7
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA AACGAATCGCACTTTTACCAC 50.0 42.9 58.0 57.5 812-824 bp 1.0 74.4