Hotspot

ndhG-ndhI

SSC · 119955-120286 · 332 bp

Pi 0.0387

MarkerSeek score 76.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 332
variable_sites: 11
pi: 0.0387
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CAAGACCCGATCCCAGAAAG	CCGTTTACCGCTGTCAGTAA	55.0	50.0	58.0	57.9	422-448 bp	1.0	85.2
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	CCGTTTACCGCTGTCAGTAA	50.0	50.0	58.0	57.9	421-447 bp	1.0	85.1
ndhG-ndhI_p3	TACAAGACCCGATCCCAGAA	CCGTTTACCGCTGTCAGTAA	50.0	50.0	57.8	57.9	424-450 bp	1.0	83.5
ndhG-ndhI_p4	ATACAAGACCCGATCCCAGA	CCGTTTACCGCTGTCAGTAA	50.0	50.0	57.5	57.9	425-451 bp	1.0	81.7
ndhG-ndhI_p5	ACCCGATCCCAGAAAGACTA	CCGTTTACCGCTGTCAGTAA	50.0	50.0	57.5	57.9	418-444 bp	1.0	81.1