Hotspot

ndhE-ndhG

SSC · 119117-119423 · 307 bp

Pi 0.049

MarkerSeek score 80.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 307
variable_sites: 12
pi: 0.049
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTTCGGCTCGTGATCAATCC	AGCTGCTTTGATAGGTGCAA	50.0	45.0	58.3	57.8	362-424 bp	1.0	82.0
ndhE-ndhG_p2	TTTCGGCTCGTGATCAATCC	GGTGCAATTGTTGTAGCTCG	50.0	50.0	58.3	57.8	349-411 bp	1.0	81.8
ndhE-ndhG_p3	CGGCTCGTGATCAATCCATA	AGCTGCTTTGATAGGTGCAA	50.0	45.0	57.6	57.8	359-421 bp	1.0	81.3
ndhE-ndhG_p4	CGGCTCGTGATCAATCCATA	GGTGCAATTGTTGTAGCTCG	50.0	50.0	57.6	57.8	346-408 bp	1.0	81.1
ndhE-ndhG_p5	AACCATATTTCGGCTCGTGA	AGCTGCTTTGATAGGTGCAA	45.0	45.0	57.3	57.8	369-431 bp	1.0	79.3