Hotspot

ccsA-ndhD

SSC · 116272-116665 · 394 bp

Back to Zornia

Pi 0.1
MarkerSeek score 76.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
394
variable_sites
35
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TCAGGAGCAGTATGGGCTAA CGAAATGGGTGGAATGGCTA 50.0 50.0 57.8 57.9 1012-1052 bp 1.0 84.7
ccsA-ndhD_p2 TTCAGGAGCAGTATGGGCTA CGAAATGGGTGGAATGGCTA 50.0 50.0 57.8 57.9 1013-1053 bp 1.0 84.7
ccsA-ndhD_p3 ACCCAAGGCTTTGCTACTTC CGAAATGGGTGGAATGGCTA 50.0 50.0 58.1 57.9 1420-1460 bp 1.0 84.2
ccsA-ndhD_p4 ACCCAAGGCTTTGCTACTTC TATGGGACTAAATGGGGCCA 50.0 50.0 58.1 58.1 1531-1571 bp 1.0 83.6
ccsA-ndhD_p5 ACCCAAGGCTTTGCTACTTC TGACGAAATGGGTGGAATGG 50.0 50.0 58.1 58.2 1423-1463 bp 1.0 83.3