Hotspot

psbK-trnQ-UUG

LSC · 55854-56670 · 817 bp

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Pi 0.0626
MarkerSeek score 73.80
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
LSC
length_bp
817
variable_sites
37
pi
0.0626
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 GCGGAGTGAATACAGACCAA GATATGCTCTGGGACGGAAG 50.0 55.0 57.6 57.6 719-935 bp 1.0 81.0
psbK-trnQ-UUG_p2 AAAGGGCGGAGTGAATACAG GATATGCTCTGGGACGGAAG 50.0 55.0 57.6 57.6 724-940 bp 1.0 80.8
psbK-trnQ-UUG_p3 AAGGGCGGAGTGAATACAGA GATATGCTCTGGGACGGAAG 50.0 55.0 58.4 57.6 723-939 bp 1.0 80.5
psbK-trnQ-UUG_p4 GCGGAGTGAATACAGACCAA GACCAAAACCCGTTGCCTTA 50.0 50.0 57.6 58.7 677-893 bp 1.0 79.3
psbK-trnQ-UUG_p5 AAAGGGCGGAGTGAATACAG GACCAAAACCCGTTGCCTTA 50.0 50.0 57.6 58.7 682-898 bp 1.0 79.1