Hotspot

psbI-psbK

LSC · 55161-55667 · 507 bp

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Pi 0.0354
MarkerSeek score 75.20
Primer available yes
Label psbI-psbK

Feature Metadata

label_name
psbI-psbK
feature_type
igs
region
LSC
length_bp
507
variable_sites
17
pi
0.0354
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-psbK_p1 GGATTACGTCCAGGATCGTT CTTTTGTTTGGCAAGCTGCT 50.0 45.0 57.1 58.1 620-637 bp 1.0 78.1
psbI-psbK_p2 GGATTACGTCCAGGATCGTT GTTTGGCAAGCTGCTGTAAG 50.0 50.0 57.1 57.9 615-632 bp 1.0 78.1
psbI-psbK_p3 TCTTCACGTCCTGGATTACG CTTTTGTTTGGCAAGCTGCT 50.0 45.0 57.1 58.1 632-649 bp 1.0 77.6
psbI-psbK_p4 TCTTCACGTCCTGGATTACG GTTTGGCAAGCTGCTGTAAG 50.0 50.0 57.1 57.9 627-644 bp 1.0 77.6
psbI-psbK_p5 GGATTACGTCCAGGATCGTT TGGCAAGCTGCTGTAAGTTT 50.0 45.0 57.1 58.3 612-629 bp 1.0 76.1