Hotspot

trnC-GCA-rpoB

LSC · 34477-35632 · 1156 bp

Back to Zornia

Pi 0.0501
MarkerSeek score 75.10
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1156
variable_sites
48
pi
0.0501
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 CCGGATTTGAACTGGGGAAA GACATTCCCGCATTTTCACC 50.0 50.0 58.1 57.7 1057-1247 bp 1.0 84.5
trnC-GCA-rpoB_p2 AAAAGGATTTGCAGTCCCCC GACATTCCCGCATTTTCACC 50.0 50.0 58.4 57.7 1039-1229 bp 1.0 82.3
trnC-GCA-rpoB_p3 GTCTATATCAGGCGACACCC GACATTCCCGCATTTTCACC 55.0 50.0 57.3 57.7 1075-1265 bp 1.0 79.2
trnC-GCA-rpoB_p4 TCTATATCAGGCGACACCCG GACATTCCCGCATTTTCACC 55.0 50.0 58.8 57.7 1074-1264 bp 1.0 79.0
trnC-GCA-rpoB_p5 GACACCCGGATTTGAACTGG GACATTCCCGCATTTTCACC 55.0 50.0 58.8 57.7 1062-1252 bp 1.0 78.4