Hotspot

psbM-petN

LSC · 32708-33563 · 856 bp

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Pi 0.0437
MarkerSeek score 77.00
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
856
variable_sites
37
pi
0.0437
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCACTGTTCATTCT TCTTGCTTGGGCTGCTTTAA 42.9 45.0 57.6 58.0 1011-1021 bp 1.0 72.3
psbM-petN_p2 TGCTACTGCACTGTTCATTCT CGTGGTATGGGGAAGAAGTG 42.9 55.0 57.6 58.0 963-973 bp 1.0 72.3
psbM-petN_p3 TGCTACTGCACTGTTCATTCT TCGTGGTATGGGGAAGAAGT 42.9 50.0 57.6 58.1 964-974 bp 1.0 72.1
psbM-petN_p4 TGCTACTGCACTGTTCATTCT AGTAAGTCTTGCTTGGGCTG 42.9 50.0 57.6 57.8 1017-1027 bp 1.0 70.8
psbM-petN_p5 TGCTACTGCACTGTTCATTCT CTCTCGTGGTATGGGGAAGA 42.9 55.0 57.6 58.2 967-977 bp 1.0 70.7