Hotspot

trnS-UGA-psbC

LSC · 26808-27001 · 194 bp

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Pi 0.0481
MarkerSeek score 78.70
Primer available yes
Label trnS-UGA-psbC

Feature Metadata

label_name
trnS-UGA-psbC
feature_type
igs
region
LSC
length_bp
194
variable_sites
9
pi
0.0481
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbC_p1 GAGAGATGGCTGAGTGGTTG TAGGTCATTTATGGCACGCG 55.0 50.0 58.0 58.4 392-393 bp 1.0 79.7
trnS-UGA-psbC_p2 GCTGAGTGGTTGATAGCTCC TAGGTCATTTATGGCACGCG 55.0 50.0 58.1 58.4 384-385 bp 1.0 79.5
trnS-UGA-psbC_p3 GGCTGAGTGGTTGATAGCTC TAGGTCATTTATGGCACGCG 55.0 50.0 58.1 58.4 385-386 bp 1.0 79.5
trnS-UGA-psbC_p4 GGTTGATAGCTCCGGTCTTG TAGGTCATTTATGGCACGCG 55.0 50.0 58.1 58.4 377-378 bp 1.0 79.5
trnS-UGA-psbC_p5 CTGAGTGGTTGATAGCTCCG TAGGTCATTTATGGCACGCG 55.0 50.0 57.8 58.4 383-384 bp 1.0 78.2