Hotspot

trnG-GCC-psbZ

LSC · 25507-26143 · 637 bp

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Pi 0.052
MarkerSeek score 81.20
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
637
variable_sites
29
pi
0.052
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA TTCCGGTACATCATTGTGGA 50.0 45.0 57.8 56.5 686-751 bp 1.0 74.2
trnG-GCC-psbZ_p2 TCTAAATAAAGGCAGGGGCG TTCCGGTACATCATTGTGGA 50.0 45.0 57.7 56.5 726-791 bp 1.0 72.7
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TCCGGTACATCATTGTGGAT 50.0 45.0 57.8 56.3 685-750 bp 1.0 72.4
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA TTTCCGGTACATCATTGTGGA 50.0 42.9 57.8 57.3 687-752 bp 1.0 72.2
trnG-GCC-psbZ_p5 TCTAAATAAAGGCAGGGGCG TCCGGTACATCATTGTGGAT 50.0 45.0 57.7 56.3 725-790 bp 1.0 70.8