Hotspot

trnfM-CAU-trnG-GCC

LSC · 25304-25435 · 132 bp

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Pi 0.0579
MarkerSeek score 77.10
Primer available yes
Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name
trnfM-CAU-trnG-GCC
feature_type
igs
region
LSC
length_bp
132
variable_sites
7
pi
0.0579
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-GCC_p1 CTTGAGGTCACGGGTTCAAA TCTTTGCCAAGGAGAAGACG 50.0 50.0 58.0 57.8 210-211 bp 1.0 85.0
trnfM-CAU-trnG-GCC_p2 TGAGGTCACGGGTTCAAATC TCTTTGCCAAGGAGAAGACG 50.0 50.0 57.8 57.8 208-209 bp 1.0 83.9
trnfM-CAU-trnG-GCC_p3 GTAGCTCGCAAGGCTCATAA TCTTTGCCAAGGAGAAGACG 50.0 50.0 57.8 57.8 231-232 bp 1.0 83.1
trnfM-CAU-trnG-GCC_p4 TAGCTCGCAAGGCTCATAAC TCTTTGCCAAGGAGAAGACG 50.0 50.0 57.8 57.8 230-231 bp 1.0 83.1
trnfM-CAU-trnG-GCC_p5 TCTCTTTATCGCGGGGTAGA TCTTTGCCAAGGAGAAGACG 50.0 50.0 57.6 57.8 259-260 bp 1.0 81.4