Hotspot

petA

LSC · 65201-66163 · 963 bp

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Pi 0.00104

MarkerSeek score 59.30

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGCAGAATCGTGGATAGGGA	AGACTAGAAAGAAGCAAAGGTCT	50.0	39.1	57.9	57.2	1162 bp	1.0	48.8
petA_p2	AGCAGAATCGTGGATAGGGA	AGAAAGAAGCAAAGGTCTTTTCA	50.0	34.8	57.9	56.8	1157 bp	1.0	46.0
petA_p3	AGCAGAATCGTGGATAGGGA	AGACTAGAAAGAAGCAAAGGTCTT	50.0	37.5	57.9	57.8	1162 bp	1.0	45.9
petA_p4	AGCAGAATCGTGGATAGGGA	AGACTAGAAAGAAGCAAAGGTC	50.0	40.9	57.9	55.8	1162 bp	1.0	45.4
petA_p5	AGCAGAATCGTGGATAGGGA	GACTAGAAAGAAGCAAAGGTCT	50.0	40.9	57.9	55.8	1161 bp	1.0	45.4