Hotspot

petA-psbJ

LSC · 66164-67369 · 1206 bp

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Pi 0.00166
MarkerSeek score 59.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1206
variable_sites
2
pi
0.00166
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTGTCCAAGGTCTCTTGTT TTCCGGATTAGGCTCATCCC 50.0 55.0 57.8 58.9 1342 bp 1.0 70.6
petA-psbJ_p2 CGTGTCCAAGGTCTCTTGTT CGGATTAGGCTCATCCCTGT 50.0 55.0 57.8 58.9 1339 bp 1.0 70.6
petA-psbJ_p3 CGTTACGTGTCCAAGGTCTC TTCCGGATTAGGCTCATCCC 55.0 55.0 58.3 58.9 1347 bp 1.0 70.1
petA-psbJ_p4 CGTTACGTGTCCAAGGTCTC CGGATTAGGCTCATCCCTGT 55.0 55.0 58.3 58.9 1344 bp 1.0 70.1
petA-psbJ_p5 GATCCGTTACGTGTCCAAGG TTCCGGATTAGGCTCATCCC 55.0 55.0 58.4 58.9 1351 bp 1.0 69.7