Hotspot

atpI-atpH

LSC · 49088-50442 · 1355 bp

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Pi 0.01

MarkerSeek score 65.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1355
variable_sites: 11
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.3	58.0	1258-1517 bp	1.0	78.4
atpI-atpH_p2	GCTGCGGCTTATATAGGTGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.3	58.0	1170-1429 bp	1.0	78.4
atpI-atpH_p3	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	45.0	50.0	57.2	58.0	1294-1553 bp	1.0	78.2
atpI-atpH_p4	ACAAGCGGTATTCAAGCTCT	ATAACGGAAGCGGCAGAAAT	45.0	45.0	57.2	58.0	1206-1465 bp	1.0	78.2
atpI-atpH_p5	GCTGCGGCTTATATAGGTGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.3	58.2	1171-1430 bp	1.0	77.3