Hotspot

trnC-GCA-rpoB

LSC · 35681-36726 · 1046 bp

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Pi 0.000957

MarkerSeek score 59.40

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 1
pi: 0.000957
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	TAAGCCCCGATCAATGAACC	50.0	50.0	58.1	57.7	1201-1202 bp	1.0	76.7
trnC-GCA-rpoB_p2	CTCTTGTTGATCAGGCGACA	TAAGCCCCGATCAATGAACC	50.0	50.0	57.9	57.7	1222-1223 bp	1.0	76.7
trnC-GCA-rpoB_p3	CCGGATTTGAACTGGGGAAA	GCCCCGATCAATGAACCTAC	50.0	55.0	58.1	58.4	1198-1199 bp	1.0	76.2
trnC-GCA-rpoB_p4	CTCTTGTTGATCAGGCGACA	GCCCCGATCAATGAACCTAC	50.0	55.0	57.9	58.4	1219-1220 bp	1.0	76.2
trnC-GCA-rpoB_p5	TCTTGTTGATCAGGCGACAC	TAAGCCCCGATCAATGAACC	50.0	50.0	58.2	57.7	1221-1222 bp	1.0	75.8