Hotspot

ycf3

LSC · 45446-47647 · 2202 bp

Back to Ziziphus

Pi 0.0121

MarkerSeek score 54.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2202
variable_sites: 73
pi: 0.0121
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACACATACAAAGAGACCCGC	TTTCTCACAACACGAAGGGT	50.0	45.0	57.9	57.3	2939-2986 bp	0.6	62.1
ycf3_p2	TTCCAAACCGTGTGAACAGT	ACACAAAACGGACGGTTAGT	45.0	45.0	57.9	57.7	2932-2993 bp	0.3	53.7
ycf3_p3	TTCCAAACCGTGTGAACAGT	CACACAACACAAAACGGACG	45.0	50.0	57.9	58.5	2938-2999 bp	0.3	52.4
ycf3_p4	TCCAAACCGTGTGAACAGTC	ACACAAAACGGACGGTTAGT	50.0	45.0	58.3	57.7	2931-2992 bp	0.3	51.9
ycf3_p5	TTCCAAACCGTGTGAACAGT	ACACACAACACAAAACGGAC	45.0	45.0	57.9	57.4	2939-3000 bp	0.3	51.2