Hotspot

ndhF

SSC · 115576-117825 · 2250 bp

Back to Ziziphus

Pi 0.0109

MarkerSeek score 42.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 78
pi: 0.0109
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	AAGCCGGTGAATCAGAAACA	50.0	45.0	58.0	57.7	2787-2820 bp	1.0	82.0
ndhF_p2	GCAGAATACCGTCACCCATT	AGCCGGTGAATCAGAAACAA	50.0	45.0	58.0	57.7	2786-2819 bp	1.0	82.0
ndhF_p3	AGGGGGACTAAACAGGAACA	AAGCCGGTGAATCAGAAACA	50.0	45.0	57.9	57.7	2674-2707 bp	1.0	81.5
ndhF_p4	AGGGGGACTAAACAGGAACA	AGCCGGTGAATCAGAAACAA	50.0	45.0	57.9	57.7	2673-2706 bp	1.0	81.5
ndhF_p5	ACAACTTCAGAAACGAGGGG	AGCCGGTGAATCAGAAACAA	50.0	45.0	57.7	57.7	2688-2721 bp	1.0	80.5