Hotspot

rps15-ycf1

SSC · 129917-130291 · 375 bp

Back to Ziziphus

Pi 0.0372

MarkerSeek score 64.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 375
variable_sites: 41
pi: 0.0372
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	TCCTCTATTCAAAGGGGCGA	50.0	50.0	58.0	58.1	1560-1583 bp	1.0	85.6
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	TGCCTCCTGCTTAGACTGAT	50.0	50.0	58.0	58.1	1602-1625 bp	1.0	85.5
rps15-ycf1_p3	AACACCATGCATTGATGGGT	ATTATCGACCCAGTCCGTCT	45.0	50.0	58.1	57.9	1333-1356 bp	0.6	69.4
rps15-ycf1_p4	TCCCAACGCGTTACATAAGG	ATTATCGACCCAGTCCGTCT	50.0	50.0	58.0	57.9	1470-1493 bp	0.6	69.3
rps15-ycf1_p5	GTTTTTCCATCCCTCGGTGT	ATTATCGACCCAGTCCGTCT	50.0	50.0	58.1	57.9	1418-1441 bp	0.5	64.3