Hotspot

ndhF-rpl32

SSC · 117826-119122 · 1297 bp

Back to Ziziphus

Pi 0.0226

MarkerSeek score 62.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1297
variable_sites: 82
pi: 0.0226
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGGAAGGGGAATGAAAGGT	CAATGCTGCCGAATATCCCT	47.6	50.0	58.1	58.1	1203-1437 bp	1.0	79.2
ndhF-rpl32_p2	GTTGGAAGGGGAATGAAAGGT	TCAATGCTGCCGAATATCCC	47.6	50.0	58.1	58.1	1204-1438 bp	1.0	79.1
ndhF-rpl32_p3	GTTGGAAGGGGAATGAAAGGT	ATGCTGCCGAATATCCCTTC	47.6	50.0	58.1	57.8	1201-1435 bp	1.0	78.4
ndhF-rpl32_p4	GTTGGAAGGGGAATGAAAGGT	AAAAGCTTTCAATGCTGCCG	47.6	45.0	58.1	58.2	1212-1446 bp	1.0	78.2
ndhF-rpl32_p5	TTGGAAGGGGAATGAAAGGT	CAATGCTGCCGAATATCCCT	45.0	50.0	56.6	58.1	1202-1436 bp	1.0	76.9