Hotspot

psbE-petL

LSC · 68672-70066 · 1395 bp

Back to Ziziphus

Pi 0.0212

MarkerSeek score 68.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1395
variable_sites: 89
pi: 0.0212
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1532-1573 bp	1.0	72.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCTGCTAGTAGAAAACCGA	50.0	42.9	57.9	57.0	1533-1574 bp	1.0	70.7
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGAA	50.0	42.9	57.9	57.0	1532-1573 bp	1.0	70.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1455-1496 bp	0.9	67.6
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1450-1491 bp	0.9	67.3