Hotspot

rbcL-accD

LSC · 60306-61097 · 792 bp

Back to Ziziphus

Pi 0.0215

MarkerSeek score 74.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 61
pi: 0.0215
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	ACAACATCGAATCGAACCGA	55.0	45.0	57.7	57.6	871-934 bp	1.0	83.8
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	CCCCCTCCTAAACAACATCG	55.0	55.0	57.7	58.0	882-945 bp	0.9	82.0
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	AACATCGAATCGAACCGACA	55.0	45.0	57.7	57.6	869-932 bp	0.9	79.7
rbcL-accD_p4	CTTGCTCGTGAGGGTAATGA	CCCCCTCCTAAACAACATCG	50.0	55.0	57.3	58.0	926-989 bp	0.9	78.9
rbcL-accD_p5	GGAGTCCTGAACTAGCTGCT	CCCCCTCCTAAACAACATCG	55.0	55.0	58.8	58.0	883-946 bp	0.9	77.9