Hotspot

trnF-GAA-ndhJ

LSC · 52093-52822 · 730 bp

Back to Ziziphus

Pi 0.015

MarkerSeek score 67.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 31
pi: 0.015
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	870-892 bp	1.0	84.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	834-856 bp	1.0	83.7
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	858-880 bp	1.0	83.0
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATGCCGGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	883-905 bp	1.0	82.9
trnF-GAA-ndhJ_p5	GAGGATGATGCGTCGTGAAT	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	894-916 bp	0.9	78.8