Hotspot

trnC-GCA-petN

LSC · 29434-30545 · 1112 bp

Back to Ziziphus

Pi 0.0197

MarkerSeek score 65.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 66
pi: 0.0197
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	GACTACCATTACAGCAGCCC	50.0	55.0	58.1	58.0	1171-1250 bp	1.0	87.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1156-1235 bp	1.0	86.5
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TCTAGAGTCCACTTCTGCCC	50.0	55.0	58.1	58.1	1220-1299 bp	1.0	86.4
trnC-GCA-petN_p4	TCACTCGAAAAGGGAAGGGA	GACTACCATTACAGCAGCCC	50.0	55.0	58.3	58.0	1260-1339 bp	1.0	85.6
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	GACTACCATTACAGCAGCCC	50.0	55.0	58.4	58.0	1189-1268 bp	1.0	85.1