Hotspot

trnR-UCU-atpA

LSC · 10884-11305 · 422 bp

Back to Ziziphus

Pi 0.062

MarkerSeek score 79.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 422
variable_sites: 64
pi: 0.062
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGCAAAGCGTCCATTGTCTA	AGGAACAGCTGGAACGTTTT	45.0	45.0	57.8	57.9	451-678 bp	1.0	90.2
trnR-UCU-atpA_p2	TGCAAAGCGTCCATTGTCTA	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.8	58.1	485-712 bp	1.0	89.9
trnR-UCU-atpA_p3	TGCAAAGCGTCCATTGTCTA	TTTACCGAGGAAGCAGAAGC	45.0	50.0	57.8	57.9	491-718 bp	1.0	89.8
trnR-UCU-atpA_p4	TGCAAAGCGTCCATTGTCTA	ATTCAGGAACAGCTGGAACG	45.0	50.0	57.8	58.2	455-682 bp	1.0	89.1
trnR-UCU-atpA_p5	AATGCAAAGCGTCCATTGTC	AGGAACAGCTGGAACGTTTT	45.0	45.0	57.6	57.9	453-680 bp	1.0	88.8