Hotspot

ndhD

SSC · 113493-115022 · 1530 bp

Back to Ziziphora

Pi 0.00288

MarkerSeek score 40.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 9
pi: 0.00288
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGAGTTGGGACCCAAAAG	ATGTACCCAATGTGTCCGTG	50.0	50.0	57.9	57.9	2291-2300 bp	1.0	79.5
ndhD_p2	TTGGAGTTGGGACCCAAAAG	ACTGCGTTGGTTGTAAGAGG	50.0	50.0	57.9	58.1	2190-2199 bp	1.0	79.4
ndhD_p3	TTGGAGTTGGGACCCAAAAG	CTGCGTTGGTTGTAAGAGGT	50.0	50.0	57.9	58.1	2189-2198 bp	1.0	79.4
ndhD_p4	GAAGCGTGGGGATCCTATTG	ATGTACCCAATGTGTCCGTG	55.0	50.0	58.1	57.9	2308-2317 bp	1.0	79.3
ndhD_p5	GAAGCGTGGGGATCCTATTG	CTGCGTTGGTTGTAAGAGGT	55.0	50.0	58.1	58.1	2206-2215 bp	1.0	79.2