Hotspot

petD-rpoA

LSC · 76623-76821 · 199 bp

Back to Ziziphora

Pi 0.00469

MarkerSeek score 55.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 2
pi: 0.00469
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGGTTGGGTATTGGTGCAA	TGAGCATTTTCGCCTAGGAG	45.0	50.0	57.9	57.7	331-348 bp	1.0	81.2
petD-rpoA_p2	TTTGGTTGGGTATTGGTGCA	TGAGCATTTTCGCCTAGGAG	45.0	50.0	57.9	57.7	332-349 bp	1.0	81.2
petD-rpoA_p3	CAGTAGCGACAACCGTCTTT	TGAGCATTTTCGCCTAGGAG	50.0	50.0	58.2	57.7	374-391 bp	1.0	79.6
petD-rpoA_p4	CCCTTTGGTTGGGTATTGGT	TGAGCATTTTCGCCTAGGAG	50.0	50.0	57.7	57.7	335-352 bp	1.0	79.3
petD-rpoA_p5	AGCGACAACCGTCTTTTTGA	TGAGCATTTTCGCCTAGGAG	45.0	50.0	58.3	57.7	370-387 bp	1.0	78.7