Hotspot

psbB-psbT

LSC · 72911-73107 · 197 bp

Back to Ziziphora

Pi 0.0186

MarkerSeek score 56.10

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 7
pi: 0.0186
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GATGTTTTTGCCGGCATTGA	GAATTTTAGGCGGTTCTCGA	45.0	45.0	57.9	55.9	376-384 bp	1.0	66.8
psbB-psbT_p2	AGATGTTTTTGCCGGCATTG	GAATTTTAGGCGGTTCTCGA	45.0	45.0	57.9	55.9	377-385 bp	1.0	66.7
psbB-psbT_p3	ATGTTTTTGCCGGCATTGAC	GAATTTTAGGCGGTTCTCGA	45.0	45.0	58.2	55.9	375-383 bp	1.0	65.8
psbB-psbT_p4	GATGTTTTTGCCGGCATTGA	GAATTTTAGGCGGTTCTCGAA	45.0	42.9	57.9	56.6	376-384 bp	1.0	64.9
psbB-psbT_p5	AGATGTTTTTGCCGGCATTG	GAATTTTAGGCGGTTCTCGAA	45.0	42.9	57.9	56.6	377-385 bp	1.0	64.8