Hotspot

psaJ-rpl33

LSC · 66226-66677 · 452 bp

Back to Ziziphora

Pi 0.00619

MarkerSeek score 51.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 6
pi: 0.00619
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	CAAAATTACCGTTACCCGCG	45.0	50.0	58.1	57.8	520-534 bp	1.0	81.4
psaJ-rpl33_p2	TGCGTTGACATTCCCCTTTT	CAAAATTACCGTTACCCGCG	45.0	50.0	58.3	57.8	519-533 bp	1.0	79.6
psaJ-rpl33_p3	CTATGGTTCGGGGCTTTAGC	CAAAATTACCGTTACCCGCG	55.0	50.0	58.4	57.8	575-589 bp	1.0	78.5
psaJ-rpl33_p4	GCTATGGTTCGGGGCTTTAG	CAAAATTACCGTTACCCGCG	55.0	50.0	58.4	57.8	576-590 bp	1.0	78.5
psaJ-rpl33_p5	ATGCGTTGACATTCCCCTTT	CCAAAATTACCGTTACCCGC	45.0	50.0	58.1	57.5	521-535 bp	1.0	78.3