Hotspot

psaI-ycf4

LSC · 59069-59518 · 450 bp

Back to Ziziphora

Pi 0.00696

MarkerSeek score 45.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 7
pi: 0.00696
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	AGGGCCCAGCAAAAATTACT	45.0	45.0	58.0	57.7	572-588 bp	1.0	80.5
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	AGGGCCCAGCAAAAATTACT	45.0	45.0	57.9	57.7	566-582 bp	1.0	79.9
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	ACTTAGTTTTCGAGACCCCG	45.0	50.0	58.0	57.3	555-571 bp	1.0	77.3
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CTTAGTTTTCGAGACCCCGT	45.0	50.0	58.0	57.3	554-570 bp	1.0	77.3
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	TGTTCTGATCGCCAACTCAT	45.0	45.0	58.0	57.2	515-531 bp	1.0	77.3