Hotspot

rbcL-accD

LSC · 56245-57003 · 759 bp

Back to Ziziphora

Pi 0.00866

MarkerSeek score 46.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 13
pi: 0.00866
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	TCGAATTAAACCACCATCTTTGC	50.0	39.1	58.0	57.9	877-886 bp	1.0	57.2
rbcL-accD_p2	CCGCTTGTGAGGTATGGAAA	TCGAATTAAACCACCATCTTTGC	50.0	39.1	57.9	57.9	843-852 bp	1.0	56.6
rbcL-accD_p3	CGTGAAGCTTGCAAATGGAG	CGAATTAAACCACCATCTTTGCA	50.0	39.1	58.0	58.2	876-885 bp	1.0	56.0
rbcL-accD_p4	CCGCTTGTGAGGTATGGAAA	CGAATTAAACCACCATCTTTGCA	50.0	39.1	57.9	58.2	842-851 bp	1.0	55.5
rbcL-accD_p5	AAGCTTGCAAATGGAGTCCT	TCGAATTAAACCACCATCTTTGC	45.0	39.1	57.7	57.9	873-882 bp	1.0	55.0