Hotspot

trnS-GCU-trnG-UCC

LSC · 8207-8924 · 718 bp

Back to Ziziphora

Pi 0.00362
MarkerSeek score 51.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
718
variable_sites
6
pi
0.00362
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 761-787 bp 1.0 73.5
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 760-786 bp 1.0 73.5
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 761-787 bp 1.0 73.4
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 760-786 bp 1.0 73.4
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 815-841 bp 1.0 72.1