Hotspot

rps16-trnQ-UUG

LSC · 5953-6842 · 890 bp

Back to Ziziphora

Pi 0.00794

MarkerSeek score 56.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 15
pi: 0.00794
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1021-1108 bp	1.0	84.0
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	963-1050 bp	1.0	83.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	45.0	55.0	58.1	57.9	976-1063 bp	1.0	83.1
rps16-trnQ-UUG_p4	AAAAATCCACAACGGGTCGT	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.3	58.0	1048-1135 bp	1.0	82.5
rps16-trnQ-UUG_p5	AAAAATCCACAACGGGTCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.3	58.1	990-1077 bp	1.0	82.0