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rps3

LSC · 80769-81488 · 720 bp

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Pi 0.00278

MarkerSeek score 35.10

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 3
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TAGGTTCTGTCGTTCCCACT	GAGTGGGCTAAGGAAACTCG	50.0	55.0	58.0	58.0	1920 bp	1.0	81.0
rps3_p2	TAGGTTCTGTCGTTCCCACT	CAAATTGGGGGAATTCGTGC	50.0	50.0	58.0	58.0	2115 bp	1.0	80.9
rps3_p3	TAGGTTCTGTCGTTCCCACT	GCAAATTGGGGGAATTCGTG	50.0	50.0	58.0	58.0	2116 bp	1.0	80.9
rps3_p4	TAGGTTCTGTCGTTCCCACT	AAGCCGAAGTCAGTAGGAGT	50.0	50.0	58.0	58.1	1648 bp	1.0	80.5
rps3_p5	TAGGTTCTGTCGTTCCCACT	TGATGAGATTCGTTGGCGTT	50.0	45.0	58.0	57.9	1803 bp	1.0	80.4