Hotspot

ndhG

SSC · 111002-111532 · 531 bp

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Pi 0.00502

MarkerSeek score 34.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 4
pi: 0.00502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGGCAGTGGATGGGTTTTAC	GTCAACAAACAATACGCGCA	50.0	45.0	58.1	58.0	1375 bp	1.0	79.8
ndhG_p2	ACCATGTTTCGGCTTGTGAT	GTCAACAAACAATACGCGCA	45.0	45.0	58.1	58.0	1570 bp	1.0	79.7
ndhG_p3	AGGGTATTTGTTGGCAGTGG	GTCAACAAACAATACGCGCA	50.0	45.0	58.1	58.0	1386 bp	0.666667	65.8
ndhG_p4	GGGTATTTGTTGGCAGTGGA	GTCAACAAACAATACGCGCA	50.0	45.0	58.1	58.0	1385 bp	0.666667	65.8
ndhG_p5	AGGGTATTTGTTGGCAGTGG	TGTGAAGTATGTGTTCGCGT	50.0	45.0	58.1	57.9	1222 bp	0.666667	64.9