Hotspot

ndhF

SSC · 102986-105190 · 2205 bp

Back to Zizania

Pi 0.00786

MarkerSeek score 48.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2205
variable_sites: 26
pi: 0.00786
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	ACGAAACAAGGGAACTGCTT	45.0	45.0	58.0	57.9	2682-2686 bp	1.0	80.5
ndhF_p2	GGGAGCGGTAATTTCATCGT	ACGAAACAAGGGAACTGCTT	50.0	45.0	58.1	57.9	2987-2991 bp	1.0	80.4
ndhF_p3	ACCCATCAATGCATGGTGTT	ACGAAACAAGGGAACTGCTT	45.0	45.0	58.1	57.9	2783-2787 bp	1.0	80.3
ndhF_p4	AATCGCGGAAAACCGAACTA	ACGAAACAAGGGAACTGCTT	45.0	45.0	57.9	57.9	2680-2684 bp	1.0	80.2
ndhF_p5	TCGCGGAAAACCGAACTATT	ACGAAACAAGGGAACTGCTT	45.0	45.0	57.9	57.9	2678-2682 bp	1.0	80.2