Hotspot

trnI-GAU-rrn16

IRa · 123831-124139 · 309 bp

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Pi 0.00647
MarkerSeek score 57.20
Primer available yes
Label trnI-GAU-rrn16

Feature Metadata

label_name
trnI-GAU-rrn16
feature_type
igs
region
IRa
length_bp
309
variable_sites
3
pi
0.00647
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16_p1 CTATCCATGTGGCTGAGAGC TAAGGAGGGGGATGCCTAAG 55.0 55.0 57.8 57.9 484-485 bp 1.0 78.4
trnI-GAU-rrn16_p2 CGCTGATGAGCACATTGAAC TAAGGAGGGGGATGCCTAAG 50.0 55.0 57.8 57.9 503-504 bp 1.0 78.3
trnI-GAU-rrn16_p3 GGCACAACGACGCAATTATC TAAGGAGGGGGATGCCTAAG 50.0 55.0 58.2 57.9 452-453 bp 1.0 78.2
trnI-GAU-rrn16_p4 CTATCCATGTGGCTGAGAGC GTAAGGAGGGGGATGCCTAA 55.0 55.0 57.8 58.2 485-486 bp 1.0 77.9
trnI-GAU-rrn16_p5 CGCTGATGAGCACATTGAAC GTAAGGAGGGGGATGCCTAA 50.0 55.0 57.8 58.2 504-505 bp 1.0 77.8