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rps3-rpl22

LSC · 81489-81539 · 51 bp

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Pi 0.08

MarkerSeek score 81.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 51
variable_sites: 6
pi: 0.08
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	TAAATTCAGACCTCGGGCTC	50.0	50.0	57.9	57.0	220-226 bp	1.0	74.1
rps3-rpl22_p2	GTGCGAACCAAAAGGAATGG	ATAAATTCAGACCTCGGGCTC	50.0	47.6	57.9	57.5	221-227 bp	1.0	70.2
rps3-rpl22_p3	GTTGTGCGAACCAAAAGGAA	TAAATTCAGACCTCGGGCTC	45.0	50.0	57.4	57.0	223-229 bp	1.0	70.1
rps3-rpl22_p4	GTGCGAACCAAAAGGAATGG	ATAAATTCAGACCTCGGGCT	50.0	45.0	57.9	56.3	221-227 bp	1.0	68.4
rps3-rpl22_p5	TGTGCGAACCAAAAGGAATG	TAAATTCAGACCTCGGGCTC	45.0	50.0	57.2	57.0	221-227 bp	1.0	68.3