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petL-petG

LSC · 65065-65249 · 185 bp

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Pi 0.0036

MarkerSeek score 29.70

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 1
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGAACAAGATACGTCTTATTTGA	ACGCAGTCACGAATAATCCC	30.4	50.0	53.8	58.1	283-284 bp	1.0	40.6
petL-petG_p2	TGAACAAGATACGTCTTATTTGA	CAGTCACGAATAATCCCGCT	30.4	50.0	53.8	57.8	280-281 bp	1.0	40.6
petL-petG_p3	TTGAACAAGATACGTCTTATTTGA	ACGCAGTCACGAATAATCCC	29.2	50.0	54.6	58.1	284-285 bp	1.0	40.6
petL-petG_p4	TGAACAAGATACGTCTTATTTGA	CAAAGATCCAACTGGTCCCC	30.4	55.0	53.8	58.2	320-321 bp	1.0	40.5
petL-petG_p5	TGAACAAGATACGTCTTATTTGA	CGCCTGTATTGCAAATACGC	30.4	50.0	53.8	58.3	299-300 bp	1.0	40.5